1 00:00:00,220 --> 00:00:03,790 and their their complaint was that's sir it it works but 2 00:00:03,790 --> 00:00:06,720 you have to like keep an eye on and make sure that it doesn't like mess up and 3 00:00:06,720 --> 00:00:07,390 these things are 4 00:00:07,390 --> 00:00:10,710 designs that they feel in the ass to get means every couple months and yet to pay 5 00:00:10,710 --> 00:00:11,690 ten grand to get them 6 00:00:11,690 --> 00:00:15,759 has done answer the huge demand for like you know things that just work and that 7 00:00:15,759 --> 00:00:17,190 are easy to fix yourself kinda thing 8 00:00:17,190 --> 00:00:22,230 anode love to see this thing just do that I own one as well I'm in the 9 00:00:22,230 --> 00:00:23,570 process of assembling at 10 00:00:23,570 --> 00:00:26,779 come on over to 11 00:00:26,779 --> 00:00:33,420 try and help me a but I'll definitely be able to assemble this robot long before 12 00:00:33,420 --> 00:00:37,000 I'm able to execute %uh a lab protocol 13 00:00:37,000 --> 00:00:42,090 by hand myself %uh without significant help from Kevin for example 14 00:00:42,090 --> 00:00:45,770 I'm there's a there's other hardware projects 15 00:00:45,770 --> 00:00:50,539 as well yet this is a bio printer I which is basically an inkjet printer 16 00:00:50,539 --> 00:00:50,829 that 17 00:00:50,829 --> 00:00:54,129 was hacked to Prince I you know biological media 18 00:00:54,129 --> 00:00:57,629 I was very yet you I am just 19 00:00:57,629 --> 00:01:02,039 bacterial cells and so this is from by a curious which is a 20 00:01:02,039 --> 00:01:06,220 I'm you i buy was facing ten San Francisco and 21 00:01:06,220 --> 00:01:10,720 this is really cool cuz I E recently found another project at Duke University 22 00:01:10,720 --> 00:01:12,270 in this is the cure the academic one 23 00:01:12,270 --> 00:01:16,570 I am and they were using a printer that could print on the CDs 24 00:01:16,570 --> 00:01:19,570 so you base their place a CD with a go you know 25 00:01:19,570 --> 00:01:23,009 petri dish and then you would be able to Prince and yourself that way 26 00:01:23,009 --> 00:01:26,700 so and I was completely independent they had no idea that this is this its allies 27 00:01:26,700 --> 00:01:29,799 talk with them the right to cancel amazing yeah 28 00:01:29,799 --> 00:01:33,150 I think you know one of the ultimate goals a 29 00:01:33,150 --> 00:01:36,170 these ideas a bio printers om is 30 00:01:36,170 --> 00:01:39,880 not so so this was like a 2d printed printed text 31 00:01:39,880 --> 00:01:43,710 on an agar plate and anger is just food for bacteria on but you know the 32 00:01:43,710 --> 00:01:45,490 ultimate goal of this kind of technology 33 00:01:45,490 --> 00:01:48,700 is to print 3d structures so for example print 34 00:01:48,700 --> 00:01:52,420 a liver print heart print the 35 00:01:52,420 --> 00:01:56,299 a leg bone or something like that home I mean only people like Steve Jobs can get 36 00:01:56,299 --> 00:01:57,780 liver transplants in time 37 00:01:57,780 --> 00:02:01,149 to survive you know terrible diseases 38 00:02:01,149 --> 00:02:05,219 a man if we could bill 39 00:02:05,219 --> 00:02:08,819 livers and hearts for people that would be fantastic your 40 00:02:08,819 --> 00:02:12,080 yes 41 00:02:12,080 --> 00:02:15,900 years but those those aren't like a organs like it like that like a liver 42 00:02:15,900 --> 00:02:19,080 has to function but yeah exactly where were we're working towards that and I 43 00:02:19,080 --> 00:02:19,720 think that 44 00:02:19,720 --> 00:02:23,200 and another cool thing with the printing aspect is that you know you can just 45 00:02:23,200 --> 00:02:24,620 print text like in this 46 00:02:24,620 --> 00:02:28,260 this picture or you know if you had to use you can imagine the guys or to 47 00:02:28,260 --> 00:02:32,290 grid love conditions weird you to scream like a drugs like 48 00:02:32,290 --> 00:02:35,290 it again you know how to printer which cost like 50 bucks 49 00:02:35,290 --> 00:02:39,050 to do this high throughput rate experiment that Sage that's huge 50 00:02:39,050 --> 00:02:42,870 %um Victor discoveries the drop like two hundred million dollars in doing 51 00:02:42,870 --> 00:02:46,250 hunting so yeah very in when you think about it 52 00:02:46,250 --> 00:02:50,220 you know medicine my dad taught tells me don't call it drugs 53 00:02:50,220 --> 00:02:53,310 when you're talking to people as it sounds that sucks medicine 54 00:02:53,310 --> 00:02:56,319 arm but you know early you know 55 00:02:56,319 --> 00:03:00,989 medicine and the drug industry is important because that's having a lot of 56 00:03:00,989 --> 00:03:02,150 our life-saving 57 00:03:02,150 --> 00:03:06,450 medicine but medicine has been around a lot longer than 58 00:03:06,450 --> 00:03:09,540 then the industrial complex a lot longer than big drug companies 59 00:03:09,540 --> 00:03:13,440 and people regular people have been discovering medicine from long long time 60 00:03:13,440 --> 00:03:13,849 ago 61 00:03:13,849 --> 00:03:18,150 and I don't see any reason why %ah you know things could change again where we 62 00:03:18,150 --> 00:03:18,810 take back 63 00:03:18,810 --> 00:03:22,209 medical research in regards to medicine or things back into you that the 64 00:03:22,209 --> 00:03:23,300 citizenry where 65 00:03:23,300 --> 00:03:26,750 a lot more people are involved we can get potentially life-saving medicine 66 00:03:26,750 --> 00:03:28,120 faster cheaper 67 00:03:28,120 --> 00:03:34,170 better quality or whenever so I'm just just think about that xanada canada 68 00:03:34,170 --> 00:03:37,470 and great hit 69 00:03:37,470 --> 00:03:41,290 I am yes so I was thinking of 70 00:03:41,290 --> 00:03:44,580 the on the rate but zombies for their therefore spaces 71 00:03:44,580 --> 00:03:47,620 going on right now ideal this one is by a space 72 00:03:47,620 --> 00:03:51,830 and and that's in Victoria BC I am 73 00:03:51,830 --> 00:03:55,590 and Mercer ID it there is a universe the 74 00:03:55,590 --> 00:03:59,140 shared space now so the public place is set up in their their spending 75 00:03:59,140 --> 00:04:02,780 i dooo Vancouver is and 76 00:04:02,780 --> 00:04:06,530 also dairy they're doing their the course of things like powered space they 77 00:04:06,530 --> 00:04:08,239 will have licked their own dedicated space yet 78 00:04:08,239 --> 00:04:11,920 but there you know gabled Maker Faires and things like that I'm 79 00:04:11,920 --> 00:04:15,750 and then pick a BIOS probably the newest one and we're also can have some later 80 00:04:15,750 --> 00:04:17,410 TI doubtfire Vancouver 81 00:04:17,410 --> 00:04:20,989 but cool product that's that they're working on now is 82 00:04:20,989 --> 00:04:24,509 doing microfluidics %uh fermentation so you know 83 00:04:24,509 --> 00:04:28,509 you'd have you make off with device and then you do your fermentation action and 84 00:04:28,509 --> 00:04:29,460 then you have a good rapport 85 00:04:29,460 --> 00:04:32,840 beers like that an which is hilarious 86 00:04:32,840 --> 00:04:37,130 a.m. so is does that mean that we can expect in five or six years that 87 00:04:37,130 --> 00:04:40,360 iPhone 8 will be able to squeeze it and beer 88 00:04:40,360 --> 00:04:43,630 will drop it yeah that more 89 00:04:43,630 --> 00:04:47,300 pure grain alcohol their medicine at I'd buy it 90 00:04:47,300 --> 00:04:51,720 and am and then do you know you know peeling or more about us writer 91 00:04:51,720 --> 00:04:55,460 yes %uh there's also doin by a Toronto which is unique in Canada 92 00:04:55,460 --> 00:04:59,100 on in that you know they have been orphaned they haven't had a space 93 00:04:59,100 --> 00:05:04,500 caller on the been working out have om a hack space they're called pack lab .to 94 00:05:04,500 --> 00:05:07,770 and because toronto is Canada's largest city 95 00:05:07,770 --> 00:05:12,039 people there you know words spread pretty fast so to put in context like 96 00:05:12,039 --> 00:05:15,669 by a space Victoria Dr Vancouver become by Montreal 97 00:05:15,669 --> 00:05:18,910 have a vote 30 or 40 members 98 00:05:18,910 --> 00:05:22,990 hour's delay about ron has about 300 members 99 00:05:22,990 --> 00:05:26,580 which is really exciting and and there's a a process now to you 100 00:05:26,580 --> 00:05:30,220 try and find these people a real home so they can get those numbers and start 101 00:05:30,220 --> 00:05:31,220 putting them to you 102 00:05:31,220 --> 00:05:35,320 work and do cool things arm and maybe you know 103 00:05:35,320 --> 00:05:39,419 do something cool on 104 00:05:39,419 --> 00:05:42,639 says being a the Inc for a printer 105 00:05:42,639 --> 00:05:45,970 I am this is a protective group called lot yes 106 00:05:45,970 --> 00:05:50,860 and their inner Paris I and they basically this is like in not 107 00:05:50,860 --> 00:05:54,150 9 genetically-engineered thing at all it's just a bacteria that can 108 00:05:54,150 --> 00:05:58,860 and you know produce this blue Inc pounds so now they're 109 00:05:58,860 --> 00:06:01,759 building this up in the gonna do it release everything I think there this 110 00:06:01,759 --> 00:06:02,520 year 111 00:06:02,520 --> 00:06:05,750 I where they'll basically sell this open 112 00:06:05,750 --> 00:06:10,650 bacteria and shares mission you know BD this kit we can grow a bunch of 113 00:06:10,650 --> 00:06:14,210 thinking pretty independent requisite stuff so serrie 114 00:06:14,210 --> 00:06:17,729 so that I D is happening then with growing groaning yeah exactly so you 115 00:06:17,729 --> 00:06:18,090 know 116 00:06:18,090 --> 00:06:21,550 this right now is that he's just mostly pens but you know how long it was a 117 00:06:21,550 --> 00:06:23,139 mistake in the pictures and then 118 00:06:23,139 --> 00:06:26,889 for japan's like it's like i mean as as we said if printer ink is so expensive 119 00:06:26,889 --> 00:06:28,800 it seems like there's a market there 120 00:06:28,800 --> 00:06:31,819 for somebody to say okay well I'm gonna growing 121 00:06:31,819 --> 00:06:34,840 at pennies for unit 122 00:06:34,840 --> 00:06:39,080 and sell it I don't know half the price a printer ink and still make a killing 123 00:06:39,080 --> 00:06:43,360 that sounds like a reasonable thing that could happen area 124 00:06:43,360 --> 00:06:48,600 heard it here first 125 00:06:48,600 --> 00:06:51,330 and just to mention another product that north or 45 this is 126 00:06:51,330 --> 00:06:54,700 from 10 space which is your new york and so 127 00:06:54,700 --> 00:06:58,900 this is a a targets and then she went to last st some teeny barcoding 128 00:06:58,900 --> 00:07:02,520 I'm which is essentially you 129 00:07:02,520 --> 00:07:06,360 fun looking you're looking for by 130 00:07:06,360 --> 00:07:09,730 listening idea its it's it's like a fingerprinting so 131 00:07:09,730 --> 00:07:13,090 year by you every organism has unique DNA 132 00:07:13,090 --> 00:07:16,700 my teen is different from that arose oMG thats 133 00:07:16,700 --> 00:07:19,790 because the genetic code tells my body had a groan when you think about it 134 00:07:19,790 --> 00:07:23,260 arose in the human are made it have generally the same 135 00:07:23,260 --> 00:07:27,280 materials a lotta Carbonneau water water sugars 136 00:07:27,280 --> 00:07:31,340 assaults various minerals and whatnot but the DNA is a program that tells our 137 00:07:31,340 --> 00:07:32,810 cellular machinery how to 138 00:07:32,810 --> 00:07:36,120 configure itself itself replicating self growing machine 139 00:07:36,120 --> 00:07:40,110 and I'm it's interesting because you know there's been some stories where you 140 00:07:40,110 --> 00:07:42,419 go to a fancy restaurant and they're selling you 141 00:07:42,419 --> 00:07:46,169 I don't know some super expensive rare 142 00:07:46,169 --> 00:07:49,530 turtle or something like that for dinner and I 143 00:07:49,530 --> 00:07:53,040 and and you like a law is this really that rare turtle 144 00:07:53,040 --> 00:07:56,270 and paying to earn dollars for this a.m. I getting my money's worth 145 00:07:56,270 --> 00:08:00,270 and it's possible using DNA barcoding to take a little 146 00:08:00,270 --> 00:08:03,600 a sample love that turtle 147 00:08:03,600 --> 00:08:07,250 and put it through a process where you extract the DNA user 148 00:08:07,250 --> 00:08:10,550 another process called PCR and women before police in reaction 149 00:08:10,550 --> 00:08:13,600 to ample fight the amount of diesel the other an amount in it that you can 150 00:08:13,600 --> 00:08:14,400 actually do some 151 00:08:14,400 --> 00:08:17,669 some now Sasan and then what you do is you %um 152 00:08:17,669 --> 00:08:21,580 ago DNA chromatograph it's like you you do process where at the end of it you 153 00:08:21,580 --> 00:08:22,490 had a picture 154 00:08:22,490 --> 00:08:26,620 up the DNA where it's like shows you these little latter 155 00:08:26,620 --> 00:08:30,100 and you can be easily compare that the same way that a a person 156 00:08:30,100 --> 00:08:34,380 forensic officer comparing IOM fingerprints with overlap the two 157 00:08:34,380 --> 00:08:37,849 arm and you do this with this DNA graph and if 158 00:08:37,849 --> 00:08:38,390 there 159 00:08:38,390 --> 00:08:42,050 if they match really well then okay yeah that's the girl they don't match 160 00:08:42,050 --> 00:08:45,390 then you can go back to that restaurant and say listen you guys are ripping 161 00:08:45,390 --> 00:08:47,170 people off on a call a newspaper 162 00:08:47,170 --> 00:08:50,690 arm and people have actually been doing this there's a story a while ago of 163 00:08:50,690 --> 00:08:53,930 some retesting fish and it turned out that the fish was in 164 00:08:53,930 --> 00:08:56,940 like tons of fish the people by restaurants is the fish that they tell 165 00:08:56,940 --> 00:08:57,490 you this 166 00:08:57,490 --> 00:09:01,329 his people can really tell on select like it. fish now because these people 167 00:09:01,329 --> 00:09:05,060 can tell the difference summer league and the you know stuff like is this 168 00:09:05,060 --> 00:09:07,600 really like you know be four scores kinda thing is some 169 00:09:07,600 --> 00:09:11,940 there's interested in these you know your stories and so the other 170 00:09:11,940 --> 00:09:15,050 same idea except you going to the wildlife and taking his bar codes and 171 00:09:15,050 --> 00:09:17,230 then locking them so you know what's our lives there 172 00:09:17,230 --> 00:09:24,230 compared between different places and so it but we have here is um 173 00:09:24,350 --> 00:09:28,540 I think that a a few things that were involved in so it's Simba is um 174 00:09:28,540 --> 00:09:31,870 it some tools and get into but what's really exciting is 175 00:09:31,870 --> 00:09:35,470 this thing called the lease in factory and it is 176 00:09:35,470 --> 00:09:38,560 a wet where product a wet where kit so 177 00:09:38,560 --> 00:09:42,310 your computer's our hardware and hardware and software 178 00:09:42,310 --> 00:09:45,839 well if we continue in in into talk and 179 00:09:45,839 --> 00:09:49,760 you know computing terms in it. terms in in biotech well then there's 180 00:09:49,760 --> 00:09:52,910 wet where recall where it's actually wet there's liquids in there 181 00:09:52,910 --> 00:09:56,890 and this is a this is a really interesting product that's come out it's 182 00:09:56,890 --> 00:09:57,140 a 183 00:09:57,110 --> 00:10:00,190 through a a partnership with genomic on and symbiotic 184 00:10:00,190 --> 00:10:03,589 and I'm here with sinbad as I'm work with them I'm 185 00:10:03,589 --> 00:10:06,640 up but what it is its a up its 186 00:10:06,640 --> 00:10:09,910 its I guess you think a bit like an Arduino for biotech in that 187 00:10:09,910 --> 00:10:14,730 is a like an R&D platform that is extensible that you can build up 188 00:10:14,730 --> 00:10:19,260 custom organisms custom microbes that will do things for you 189 00:10:19,260 --> 00:10:24,040 and this one is a kit that comes with everything you need all these DNA parts 190 00:10:24,040 --> 00:10:25,800 and an assembly protocol 191 00:10:25,800 --> 00:10:30,690 that allows you to create a micro but I P E coli that produces a product called 192 00:10:30,690 --> 00:10:31,630 view leeson 193 00:10:31,630 --> 00:10:35,910 and anyone heard of the lease in before yet gotten 194 00:10:35,910 --> 00:10:39,230 no one knows about this probably one of the reasons no one knows about it is no 195 00:10:39,230 --> 00:10:41,220 one talks about it in reason on talks about it 196 00:10:41,220 --> 00:10:44,890 because it cost $350 million dollars per kilo 197 00:10:44,890 --> 00:10:48,990 and it's sold by a company called sigma-aldrich 198 00:10:48,990 --> 00:10:52,230 and I its purchased by researchers 199 00:10:52,230 --> 00:10:55,250 oMG who are working trying to find Oct 200 00:10:55,250 --> 00:10:59,120 cheers therapies for cancer and so this 201 00:10:59,120 --> 00:11:03,000 is a it's not a drug it's not a mess in its research compound 202 00:11:03,000 --> 00:11:07,360 that is on into the process of figuring okays is something that we can use for 203 00:11:07,360 --> 00:11:11,089 for cancer therapeutics but the problem is researchers 204 00:11:11,089 --> 00:11:13,420 it's very difficult for them to get their hands on it is just so darn 205 00:11:13,420 --> 00:11:14,110 expensive 206 00:11:14,110 --> 00:11:18,810 and so so what we did at Sinbad and the genomic on we decided to take their wet 207 00:11:18,810 --> 00:11:19,870 where cat platform 208 00:11:19,870 --> 00:11:23,250 called the genomic on kit which they originally had 209 00:11:23,250 --> 00:11:27,600 that allows you to create hi these these pictures that we should before very 210 00:11:27,600 --> 00:11:28,510 similar to this where 211 00:11:28,510 --> 00:11:32,370 you can have your hello world in program your E-coli to express different 212 00:11:32,370 --> 00:11:34,190 proteins that are different colors 213 00:11:34,190 --> 00:11:37,949 we thought okay well that's cool but what's the next step our people building 214 00:11:37,949 --> 00:11:41,060 modules like Arduino shields for this product 215 00:11:41,060 --> 00:11:44,779 and they said no knowns and the same okay well we want to build the first one 216 00:11:44,779 --> 00:11:46,420 and you want to do this delay something 217 00:11:46,420 --> 00:11:50,720 and so what we've done is created this kit %uh 218 00:11:50,720 --> 00:11:54,350 it sells for for 550 bucks 219 00:11:54,350 --> 00:11:57,850 Canadian arm and we've sold about 20 of them I 220 00:11:57,850 --> 00:12:01,250 to YouTube people and stayed stable in Canada 221 00:12:01,250 --> 00:12:05,160 people in Europe arm and what they're doing is they are building this kit 222 00:12:05,160 --> 00:12:09,699 because its modular they are putting the kit together in unique ways to see 223 00:12:09,699 --> 00:12:14,279 if they can come up with that the design that produces the most 224 00:12:14,279 --> 00:12:17,440 velly since so to get the most bang for your buck added this product 225 00:12:17,440 --> 00:12:20,959 and there was a student I maybe I can 226 00:12:20,959 --> 00:12:25,680 I can find it here there's a student from Canada name 227 00:12:25,680 --> 00:12:29,970 a hundred system and he bought one of these kids he's a grad student 228 00:12:29,970 --> 00:12:33,470 working biology and his PI 229 00:12:33,470 --> 00:12:36,770 Adam working on a project that he was a super keen on but he 230 00:12:36,770 --> 00:12:41,410 he got when this product he bought it let's see if I can find here 231 00:12:41,410 --> 00:12:45,790 treason samples and he built the kit 232 00:12:45,790 --> 00:12:50,110 a multiple times in changing the the design of the DNA 233 00:12:50,110 --> 00:12:56,230 here and he actually came up with the design 234 00:12:56,230 --> 00:13:00,390 I that was very very good I'm and he so this is 235 00:13:00,390 --> 00:13:05,059 this is his basically his lab book that he's made open to the world so anyone 236 00:13:05,059 --> 00:13:06,720 can go in there and look at 237 00:13:06,720 --> 00:13:10,689 his DNA designs arm and also give them access to the tools that he used to do 238 00:13:10,689 --> 00:13:11,089 it 239 00:13:11,089 --> 00:13:15,400 which is totally not how big pharma does things 240 00:13:15,400 --> 00:13:19,339 at all on but he let's let's see if we can look at his daily here so 241 00:13:19,339 --> 00:13:21,699 transformation results 242 00:13:21,699 --> 00:13:26,500 think this one has some cool pictures so here we go so here here was a control 243 00:13:26,500 --> 00:13:29,600 these ones didn't work then %uh purple on these ones are green for yellow 244 00:13:29,600 --> 00:13:29,959 rather 245 00:13:29,959 --> 00:13:33,760 these ones didn't work these ones didn't work those ones didn't work I'll 246 00:13:33,760 --> 00:13:38,279 here's some yours some purple and blue purple really sinister plot was called 247 00:13:38,279 --> 00:13:41,880 by libya leeson if it's purple than you know the the medical 248 00:13:41,880 --> 00:13:45,100 compounds there com and so he wound up 249 00:13:45,100 --> 00:13:49,110 other similar ones near but anyways he wound up designing a genetic 250 00:13:49,110 --> 00:13:52,209 circuit that did a very good job this 251 00:13:52,209 --> 00:13:55,260 and he's now in the process love figuring out how he can 252 00:13:55,260 --> 00:13:58,920 extract the lease in and distillate and and selling 253 00:13:58,920 --> 00:14:02,209 he's a young guy I think he's slick 28 years to kids 254 00:14:02,209 --> 00:14:06,839 he's in school and he made some cash and he's really motivated to be like a 255 00:14:06,839 --> 00:14:10,220 why why is sigma-aldrich the only coming to cancel this 256 00:14:10,220 --> 00:14:13,459 I'm gonna go ahead and do it arm and we're really excited about this because 257 00:14:13,459 --> 00:14:17,220 it serve a proof-of-concept wears like you know there's a huge boom in 258 00:14:17,220 --> 00:14:20,160 computing because people like hey wait a minute this is accessible 259 00:14:20,160 --> 00:14:24,120 and I can make a tight cash doing this and I can do a lot of good I can 260 00:14:24,120 --> 00:14:28,319 make a valuable product for people arm and and life science biotech 261 00:14:28,319 --> 00:14:31,589 is is with their like this is truly happening now 262 00:14:31,589 --> 00:14:34,890 arm and certainly by the next hope there's gonna be people up here 263 00:14:34,890 --> 00:14:40,480 showing off their files I love THC 264 00:14:40,480 --> 00:14:43,600 viagara calls like that yet 265 00:14:43,600 --> 00:14:48,020 dynamite you awesome young asian countries that have been impressive just 266 00:14:48,020 --> 00:14:52,029 with the RGB which the to make you know red in blue 267 00:14:52,029 --> 00:14:55,069 I victory that glow I would force a protein 268 00:14:55,069 --> 00:14:58,319 and it's like designed in such a way that's it's not at all like the 269 00:14:58,319 --> 00:14:59,319 traditional 270 00:14:59,319 --> 00:15:02,870 I you know biochemistry molecular biology techniques doing it 271 00:15:02,870 --> 00:15:06,559 and installation is simply I'm 272 00:15:06,559 --> 00:15:09,740 a picture but either way the halls of the point is is very you know 273 00:15:09,740 --> 00:15:13,309 user friendly kinda thing I've doing the assembly up the DNA 274 00:15:13,309 --> 00:15:16,689 I mean you can look up the details on how it works and I could go through it 275 00:15:16,689 --> 00:15:17,140 but 276 00:15:17,140 --> 00:15:21,339 knaves like larry has a beber a.m. 277 00:15:21,339 --> 00:15:26,260 yet ISA you'll get get the 10 friends and experts in like 20 bucks and buy one 278 00:15:26,260 --> 00:15:26,980 is getting 279 00:15:26,980 --> 00:15:31,470 have some fun and you can go on and and I look at other people's work 280 00:15:31,470 --> 00:15:35,500 and if you dislike flight at wanna make me feel AC don't wanna do experiments 281 00:15:35,500 --> 00:15:39,399 just follow the other person's protocol annual you know by the end of two weeks 282 00:15:39,399 --> 00:15:42,770 you'll probably have like 20 grand with Avi leeson 283 00:15:42,770 --> 00:15:46,520 I in your fridge gonna have to find a way to extracted 284 00:15:46,520 --> 00:15:51,339 and legally sell it 285 00:15:51,339 --> 00:15:54,740 but you know those are cool I mean we're hackers rate you guys gotta fear at 286 00:15:54,740 --> 00:15:55,300 these 287 00:15:55,300 --> 00:16:00,309 gotta figure out these problems because they're the opportunities there 288 00:16:00,309 --> 00:16:03,589 and service the product and working on in this in my ass others being a tease 289 00:16:03,589 --> 00:16:04,550 the SE 290 00:16:04,550 --> 00:16:08,010 just mentioned I am yes so 291 00:16:08,010 --> 00:16:12,890 Serena trimix East I can produce THC and 292 00:16:12,890 --> 00:16:15,970 and yet and it will bring to do something with it and 293 00:16:15,970 --> 00:16:19,020 poly X Factor for 294 00:16:19,020 --> 00:16:23,079 on 295 00:16:23,079 --> 00:16:25,870 me it's super funny to think about what you're gonna wear imagination can go 296 00:16:25,870 --> 00:16:28,350 with this kinda thing like you know is this 297 00:16:28,350 --> 00:16:31,670 you know I do is from sickle products but you know its features is 298 00:16:31,670 --> 00:16:36,069 same time so on what's really cool about using yeast and thinking about 299 00:16:36,069 --> 00:16:39,949 East as a microbial factory when you think the factories 300 00:16:39,949 --> 00:16:43,449 their huge multi-million dollar 100 million dollar billion dollar 301 00:16:43,449 --> 00:16:47,649 machines their architectural that are owned by big companies 302 00:16:47,649 --> 00:16:51,410 arm and they're doing similar things that biology does biology are machines 303 00:16:51,410 --> 00:16:53,399 that grow things and make things right 304 00:16:53,399 --> 00:16:57,120 and so if we can find a way to program or or hijack East 305 00:16:57,120 --> 00:17:00,189 to produce other valuable products aside from 306 00:17:00,189 --> 00:17:04,610 puffy bread and beer then that's really exciting right because think about it 307 00:17:04,610 --> 00:17:05,909 you know how long have 308 00:17:05,909 --> 00:17:10,880 humans been making beer and wine 10,000 years fifteen thousand years 309 00:17:10,880 --> 00:17:13,560 twenty thousand years I don't know a long time definitely definitely 310 00:17:13,560 --> 00:17:15,120 thousands of years for sure 311 00:17:15,120 --> 00:17:18,159 people have been using a East as as 312 00:17:18,159 --> 00:17:21,220 as a tool to turn inexpensive products like 313 00:17:21,220 --> 00:17:25,089 sugary water grape juice into a high-value 314 00:17:25,089 --> 00:17:28,059 fantastically exciting product 315 00:17:28,059 --> 00:17:32,460 like like wine you know how some wine is hundreds of dollars for a moderate 316 00:17:32,460 --> 00:17:35,899 but it's just so great use to start and it's these microbes that 317 00:17:35,899 --> 00:17:39,409 eat the sugar PO wine 318 00:17:39,409 --> 00:17:43,740 and then we drink it right and soaks up its cool but when he think I think about 319 00:17:43,740 --> 00:17:44,630 how they make beer 320 00:17:44,630 --> 00:17:48,100 beers mean small batches anyone here maybe a small bash in their garage or 321 00:17:48,100 --> 00:17:49,009 basement rhetorical 322 00:17:49,009 --> 00:17:53,529 awesome me too anyone here may be really huge large factory owned by the batter 323 00:17:53,529 --> 00:17:54,240 Moulson 324 00:17:54,240 --> 00:17:57,920 wall with a brewmaster in the house 325 00:17:57,920 --> 00:18:01,890 cool awesome but but those machines cost millions hundreds millions of dollars 326 00:18:01,890 --> 00:18:02,630 but you get to make 327 00:18:02,630 --> 00:18:07,040 beer at scale and sell make big bucks but when it comes down to it the process 328 00:18:07,040 --> 00:18:08,150 is exactly the same 329 00:18:08,150 --> 00:18:11,570 right you can it's it's a totally scaleable technology 330 00:18:11,570 --> 00:18:14,600 and if we can use that idea net 331 00:18:14,600 --> 00:18:18,400 thinking to produce other products in addition to THC like 332 00:18:18,400 --> 00:18:21,650 any other kind of biological product if that we can do it that way 333 00:18:21,650 --> 00:18:25,590 that's a really to me is a really exciting idea really potentially 334 00:18:25,590 --> 00:18:30,180 super duper lucrative idea yet and he just think about plants themselves as 335 00:18:30,180 --> 00:18:30,650 like 336 00:18:30,650 --> 00:18:33,880 you know be able to piece is common on their own a plant is the key couple used 337 00:18:33,880 --> 00:18:34,530 to grow but 338 00:18:34,530 --> 00:18:37,820 if you think about Eastin this exponentially growing 339 00:18:37,820 --> 00:18:41,180 thing you can get that same amount biomass like a week for 22 years a 340 00:18:41,180 --> 00:18:41,840 pro-growth 341 00:18:41,840 --> 00:18:46,310 so and so this is the end of the beginning 342 00:18:46,310 --> 00:18:50,840 arm we're gonna put a challenge out so I've been reading 2600 the hacker 343 00:18:50,840 --> 00:18:54,480 quarterly since I was 12 or 13 or something like that arm 344 00:18:54,480 --> 00:18:58,660 and and in this whole time I mean I've missed a few issues here and then 345 00:18:58,660 --> 00:19:03,130 here and there I but I've never seen by attacking article and 2600 346 00:19:03,130 --> 00:19:06,280 has head has there been a by having our content 601 347 00:19:06,280 --> 00:19:10,620 yes fair well there needs to be more his I 348 00:19:10,620 --> 00:19:13,640 an easy more so for example is anyone familiar with 349 00:19:13,640 --> 00:19:16,910 riley's by ok older yeah it's pretty cool 350 00:19:16,910 --> 00:19:20,760 it's a to bring anything they've had 45 issues now 351 00:19:20,760 --> 00:19:24,610 om and basically if you you can download for free arm 352 00:19:24,610 --> 00:19:28,940 these guys are clearly fans a 2600 cuz if you look at the layout and the kind 353 00:19:28,940 --> 00:19:29,520 of Content 354 00:19:29,520 --> 00:19:32,950 it's very similar to a 6-under back accordingly but it's for bio 355 00:19:32,950 --> 00:19:36,220 comment if you read their mass and it says rip the start a revolution that 356 00:19:36,220 --> 00:19:39,690 will transform our lives as radically is a computer revolution in the seventies 357 00:19:39,690 --> 00:19:43,070 the biological revolution will touch every aspect of our lives 358 00:19:43,070 --> 00:19:47,180 food and health certainly but also art recreation law business 359 00:19:47,180 --> 00:19:50,700 and much more and so to me that sounds like a really cool arm 360 00:19:50,700 --> 00:19:54,280 we to stoke some fire so I'm hoping that weekend some 361 00:19:54,280 --> 00:19:57,670 you know make sure that the 2600 community also 362 00:19:57,670 --> 00:20:01,840 kinda participate in this as well and doesn't let Riley own the whole space 363 00:20:01,840 --> 00:20:05,690 on and I guess a as expanding on the idea Vienna 364 00:20:05,690 --> 00:20:09,590 the beginning in you know the hackers the by hackers this is that 365 00:20:09,590 --> 00:20:13,040 sorted out few years ago where more aware 366 00:20:13,040 --> 00:20:16,120 you know what they were doing and how this late appear to like security 367 00:20:16,120 --> 00:20:17,160 agencies things like that 368 00:20:17,160 --> 00:20:21,420 or surpass that point where now all the Securities know that this exists and the 369 00:20:21,420 --> 00:20:25,510 are you know where did and they're not you know no one's afraid to be targeted 370 00:20:25,510 --> 00:20:25,910 anymore 371 00:20:25,910 --> 00:20:31,080 basically I'm ands now people are going well as supporting the crowdfunding 372 00:20:31,080 --> 00:20:34,080 and even you miss minutes municipal governments are they 373 00:20:34,080 --> 00:20:37,880 you know seeing this as like a really good thing I got this is gonna Jerry 374 00:20:37,880 --> 00:20:42,080 capitalist through so many of them and you look at these is going and so 375 00:20:42,080 --> 00:20:45,920 in essence is up more support going for it now then there was before 376 00:20:45,920 --> 00:20:49,040 on and the coolest couple the quality is 377 00:20:49,040 --> 00:20:52,260 I I was looking at cribs in today I'm the crib Sanford 378 00:20:52,260 --> 00:20:55,880 is no caption available devices yet and you know if you have an expiry 379 00:20:55,880 --> 00:20:58,900 you can sequence and find out exactly out worse the deck level 380 00:20:58,900 --> 00:21:02,460 on and another caller ID on the corruption thing as we just saw 381 00:21:02,460 --> 00:21:03,240 something about 382 00:21:03,240 --> 00:21:07,300 DNA testing people and San others it may be a demand for 383 00:21:07,300 --> 00:21:11,610 a way to you encrypt your own DNA sled people can scan you 384 00:21:11,610 --> 00:21:14,890 some like that or or so or stand like a basically just 385 00:21:14,890 --> 00:21:19,410 get everyone CNN just put it all over the place yeah Etsy can imagine Michael 386 00:21:19,410 --> 00:21:22,450 like a weed spray thing they just take but to people's lives in 387 00:21:22,450 --> 00:21:25,800 anything this love you have a dozen different people and 388 00:21:25,800 --> 00:21:28,830 and it no that's coming up so I 389 00:21:28,830 --> 00:21:33,240 two weeks ago I was at MIT and I was China some people talking about 390 00:21:33,240 --> 00:21:36,340 you know some stuff since we actually doing science sector and I 391 00:21:36,340 --> 00:21:39,240 a guy said all this is awesome let me show you what I'm working on these just 392 00:21:39,240 --> 00:21:40,440 that finishes PhD 393 00:21:40,440 --> 00:21:44,140 and he's working on a basically say it's a it's a 394 00:21:44,140 --> 00:21:47,660 device it's about this size you know the sizeof I forever 395 00:21:47,660 --> 00:21:50,820 and it said DNA synthesizer meaning it's a 396 00:21:50,820 --> 00:21:54,430 it's a tool that actually creates DNA in the city one thing we didn't talk about 397 00:21:54,430 --> 00:21:59,840 quite yet is DNA synthesis on but so it's the opposite up DNA sequencing 398 00:21:59,840 --> 00:22:02,890 everyone's for DNA sequencing where you read in your 399 00:22:02,890 --> 00:22:06,190 genetic code and at the end of the day you've got a text file with your genetic 400 00:22:06,190 --> 00:22:08,700 code on it but we have the technology to date actually 401 00:22:08,700 --> 00:22:13,640 printed that text file arm into a real piece of DNA that you can put into an 402 00:22:13,640 --> 00:22:14,110 organism 403 00:22:14,110 --> 00:22:17,200 on and these are BigMachines you know the size of a 404 00:22:17,200 --> 00:22:21,020 free edger are free agent are getting smaller and smaller and I actually saw 405 00:22:21,020 --> 00:22:25,240 a guy holding his hand a microfluidic parade that was for the size of an 406 00:22:25,240 --> 00:22:25,820 iPhone 407 00:22:25,820 --> 00:22:31,600 it works you can print DNA on it it runs off a nine volt battery 408 00:22:31,600 --> 00:22:35,470 and I it like I am how much like that's a prototype its prolly prices 409 00:22:35,470 --> 00:22:39,889 but it was just me and acrylic probably five bucks worth of stuff 410 00:22:39,889 --> 00:22:43,119 so it's coming and lookin to legal 411 00:22:43,119 --> 00:22:46,929 here cyclical arm 412 00:22:46,929 --> 00:22:50,950 you can make viruses yeah it won't be 413 00:22:50,950 --> 00:22:54,499 as easy as anyone can imagine you know 414 00:22:54,499 --> 00:22:58,009 Maki you this is like the you know your stuff that people getting over now is a 415 00:22:58,009 --> 00:22:58,759 quick sec me know 416 00:22:58,759 --> 00:23:02,830 oh my god in the fires is but like really no likes you 417 00:23:02,830 --> 00:23:06,330 a youth you'd probably die before yes got to that point anything 418 00:23:06,330 --> 00:23:10,019 I you you're you're putting yourself at risk more than anyone else 419 00:23:10,019 --> 00:23:13,419 and so as far as that that's like 420 00:23:13,419 --> 00:23:16,749 biosafety basically I am and 421 00:23:16,749 --> 00:23:19,889 just doing the site to sell for like to note is producing the spires isn't 422 00:23:19,889 --> 00:23:23,539 its not even there yet leading edge science in what month 423 00:23:23,539 --> 00:23:27,259 one thing that was mean only started setting up bar doin by a group 424 00:23:27,259 --> 00:23:30,989 one other hurdles that we had was that we didn't know what the law was about 425 00:23:30,989 --> 00:23:31,859 biosafety 426 00:23:31,859 --> 00:23:36,289 and biosecurity we just knew that you know we did have to take some steps to 427 00:23:36,289 --> 00:23:37,559 make sure that things were safe 428 00:23:37,559 --> 00:23:41,090 but we didn't know what that was and it was difficult to connect with 429 00:23:41,090 --> 00:23:45,230 you know people in government or whoever that actually had that 430 00:23:45,230 --> 00:23:48,299 arm and and so you know years later today 431 00:23:48,299 --> 00:23:51,239 now we do know and what's interesting is the kinda stuff that's coming into 432 00:23:51,239 --> 00:23:53,580 virtually all DIY Biolabs 433 00:23:53,580 --> 00:23:57,029 isn't even on the radar of up by security people 434 00:23:57,029 --> 00:24:01,019 no one is working with human pathogens no one is working with buyers is 435 00:24:01,019 --> 00:24:05,340 the kinda stuff that people are doing our with lab E-coli 436 00:24:05,340 --> 00:24:09,799 East those kind of things that are specifically designed to work 437 00:24:09,799 --> 00:24:14,919 in a very specific environment if those microbes were to leave the petri dish 438 00:24:14,919 --> 00:24:18,340 they would not survive so it's kinda like um 439 00:24:18,340 --> 00:24:21,739 there's actually the scene in jurassic park it earlier on scene where 440 00:24:21,739 --> 00:24:26,009 I am you know they've engineered the dinosaurs to lack a certain 441 00:24:26,009 --> 00:24:29,350 enzymes that they can't exist without leaving their special feed 442 00:24:29,350 --> 00:24:33,940 thats basically exactly how lab microorganisms work they've been 443 00:24:33,940 --> 00:24:34,929 engineered to be 444 00:24:34,929 --> 00:24:39,210 week yeah exactly 445 00:24:39,210 --> 00:24:42,809 guys but as you know the yet yet that's a movie 446 00:24:42,809 --> 00:24:47,779 but you know hey to it's a fantastic movie 447 00:24:47,779 --> 00:24:50,889 we should we should all watch it we shall watch it yet 448 00:24:50,889 --> 00:24:54,710 I galleons for anything just forget all the heightened yet tackles bio 449 00:24:54,710 --> 00:24:57,720 but yet to have a dinosaur you can make you know the jerseys 450 00:24:57,720 --> 00:25:01,499 thats skin died Andrea 451 00:25:01,499 --> 00:25:06,169 in the wild joins Mike question began to question serious 452 00:25:06,169 --> 00:25:09,220 and for II 453 00:25:09,220 --> 00:25:12,599 to few more things I have a couple just couple ideas are like you know 454 00:25:12,599 --> 00:25:16,320 hope we can inspire guesses are working on anything on 455 00:25:16,320 --> 00:25:21,769 there is Lake well you guys are always find the most mileage that a No 456 00:25:21,769 --> 00:25:25,279 for one like I was tired positions in touch today and makes 457 00:25:25,279 --> 00:25:28,559 you guys should really so they can send the stuff and 458 00:25:28,559 --> 00:25:32,549 you get involved find your local by actor spacers you know I am 459 00:25:32,549 --> 00:25:36,919 star one if there is one I am and the offer free to reach out to the community 460 00:25:36,919 --> 00:25:40,340 go to do.i bile duct work is accused me of their deaths just 461 00:25:40,340 --> 00:25:43,570 no really and inspiring as far as getting stuff going 462 00:25:43,570 --> 00:25:46,619 as a couple things ideas that I once the app in our 463 00:25:46,619 --> 00:25:50,080 one the city a Programmer's Guide coding in bio 464 00:25:50,080 --> 00:25:53,679 on and I was inspired by the non from eros guide to 465 00:25:53,679 --> 00:25:57,090 trading in Python but did that and got the voicemail introductory computer 466 00:25:57,090 --> 00:25:57,639 science course 467 00:25:57,639 --> 00:26:02,809 I'm fish so and that's awesome and I once like that we're doing bio 468 00:26:02,809 --> 00:26:05,999 and nice thing that I wanna see happen is that your computer 469 00:26:05,999 --> 00:26:09,869 controls its own temperature it's you know she did from overheating things 470 00:26:09,869 --> 00:26:10,269 like that 471 00:26:10,269 --> 00:26:13,859 I wanna see somebody puts bacteria in your computer and treated by reactor 472 00:26:13,859 --> 00:26:14,970 where you can meeting 473 00:26:14,970 --> 00:26:18,230 this consistent temperature and it wasn't a cherry on it so 474 00:26:18,230 --> 00:26:22,369 sunny know do these things and take to get the pictures as me and I'll 475 00:26:22,369 --> 00:26:25,669 cause mines something like that on a I'm 476 00:26:25,669 --> 00:26:30,090 yet that's that's I guess I don't say anything else 477 00:26:30,090 --> 00:26:33,659 on yeah i think i think thats a good introduction 478 00:26:33,659 --> 00:26:36,590 army don't wanna blow your minds too much to get started but yet the 479 00:26:36,590 --> 00:26:38,200 resources are all their online 480 00:26:38,200 --> 00:26:41,659 connect with us if you have any questions comments or or 481 00:26:41,659 --> 00:26:46,429 whatever but I guess maybe we can start taking some questions now i spose 482 00:26:46,429 --> 00:26:51,559 yep the returns may come 483 00:26:51,559 --> 00:26:58,029 were there but none there 484 00:26:58,029 --> 00:27:01,470 there's arms so my question was around 485 00:27:01,470 --> 00:27:04,720 the sequencing various life forms 486 00:27:04,720 --> 00:27:08,360 genetic sequences just the identification potentially a 487 00:27:08,360 --> 00:27:12,240 functional components have those ok I know trying to T 488 00:27:12,240 --> 00:27:16,230 you know the raw data and then saying okay well then this piece is actually 489 00:27:16,230 --> 00:27:17,299 doing this 490 00:27:17,299 --> 00:27:20,490 and so forth you know kinda I guess in a way 491 00:27:20,490 --> 00:27:23,840 you know developing an old an open source toolkit love those genetic 492 00:27:23,840 --> 00:27:27,360 building blocks right because it's one thing to say all right well we can take 493 00:27:27,360 --> 00:27:31,760 you know the complete sequence from this glowing being went into this E coli 494 00:27:31,760 --> 00:27:35,029 no clothes and that's cool but you know can you take just 495 00:27:35,029 --> 00:27:38,110 a subset of that and you know transferred 496 00:27:38,110 --> 00:27:41,690 I guess the question is around have there been any efforts to 497 00:27:41,690 --> 00:27:46,789 compile a sec that kinda data and make it available in an open source fashion 498 00:27:46,789 --> 00:27:49,730 yet yet the answer is yes 499 00:27:49,730 --> 00:27:53,399 and muster which once yeah I mean this is 500 00:27:53,399 --> 00:27:56,769 a bunch I'm Desire X is actually one is them 501 00:27:56,769 --> 00:27:59,340 problems up there when I think there is another one that sidelined today a 502 00:27:59,340 --> 00:28:00,260 camera and beaded 503 00:28:00,260 --> 00:28:04,399 on but sad yet parts that I dot org is the one that's 504 00:28:04,399 --> 00:28:07,450 run by the international agenda the engineer sheen 505 00:28:07,450 --> 00:28:11,529 competition I'm item yet so 506 00:28:11,529 --> 00:28:14,769 I am this is going to surf around and look at what 507 00:28:14,769 --> 00:28:18,980 parts have been submitted and the system in soviet the way it works is that it's 508 00:28:18,980 --> 00:28:22,559 undergraduate research teams that just work on a project for the summer and 509 00:28:22,559 --> 00:28:25,990 you know 'cause in DNA but in the experience if it works and then summit 510 00:28:25,990 --> 00:28:26,809 1382 511 00:28:26,809 --> 00:28:30,130 the registry I am answer right now I think they just 512 00:28:30,130 --> 00:28:33,299 they opened up this year to do I buy labs I'm hands 513 00:28:33,299 --> 00:28:37,120 the way works that you can just the sand you first like it stack a party 514 00:28:37,120 --> 00:28:40,649 news and then you can request a price from the registry I'm in to see the case 515 00:28:40,649 --> 00:28:42,070 %uh me this thing that kills 516 00:28:42,070 --> 00:28:46,139 am you know for TCL genuine their CTS used in there yet 517 00:28:46,139 --> 00:28:49,289 I'm innings be others other 518 00:28:49,289 --> 00:28:53,130 this other repositories like the NCBI which is open database that you can go 519 00:28:53,130 --> 00:28:53,580 and 520 00:28:53,580 --> 00:28:57,000 search for various organisms and there's a there's a difference where you know 521 00:28:57,000 --> 00:29:01,419 some organisms that are %uh research organisms are 522 00:29:01,419 --> 00:29:05,139 very highly characterize people know a lot about it you can go to the database 523 00:29:05,139 --> 00:29:06,200 and find an organism 524 00:29:06,200 --> 00:29:09,779 where we have the genetic sequence but no one knows with the parts do 525 00:29:09,779 --> 00:29:13,960 no one knows what the parts are whenever I'm sometimes people what they do is 526 00:29:13,960 --> 00:29:14,419 they 527 00:29:14,419 --> 00:29:18,700 on they compare known parts to in an organism see if their similarities 528 00:29:18,700 --> 00:29:23,240 and select pic okay this roses red and here's his other Florence red okay well 529 00:29:23,240 --> 00:29:23,690 maybe 530 00:29:23,690 --> 00:29:28,110 it's similar com but a lot it was interesting about genetic ozell X 531 00:29:28,110 --> 00:29:29,750 software is written by people 532 00:29:29,750 --> 00:29:32,909 we've we invented computers reinvented 533 00:29:32,909 --> 00:29:37,360 software I'm whereas DNA is our life is 534 00:29:37,360 --> 00:29:40,429 pre-existing we did not admit it and so it's not really a 535 00:29:40,429 --> 00:29:44,029 a process %uh engineering it's a process of 536 00:29:44,029 --> 00:29:47,620 discovery on which is a little bit different which is interesting cuz its 537 00:29:47,620 --> 00:29:51,389 I think maybe that could be reason as to why we see such a a different arm 538 00:29:51,389 --> 00:29:54,559 audience of people there are involved in by hacking versus 539 00:29:54,559 --> 00:29:58,340 straight up computing I don't know if anyone would argue with me but when I 540 00:29:58,340 --> 00:30:01,950 was and so in computer science ninety percent of the people my class were guys 541 00:30:01,950 --> 00:30:06,320 om but that's not the case with bio when I was in bio class is it was 542 00:30:06,320 --> 00:30:10,880 50/50 or maybe even suede I more towards female side which is going to be 543 00:30:10,880 --> 00:30:15,740 interesting arm opportunity for a whole new group of people to get involved in 544 00:30:15,740 --> 00:30:16,860 this evidentiary 545 00:30:16,860 --> 00:30:20,809 firms from an explosion 546 00:30:20,809 --> 00:30:24,740 arm so I'm interested with the on 547 00:30:24,740 --> 00:30:30,490 talking about decrease in cost of production for drugs 548 00:30:30,490 --> 00:30:34,090 and how that relates to pounds because I know that one of the frequently raised 549 00:30:34,090 --> 00:30:37,879 arguments for through maintaining a patent system all 550 00:30:37,879 --> 00:30:41,659 is that it's such a high cost am also interested 551 00:30:41,659 --> 00:30:46,899 he say that a lot of these furtive tools are becoming small an open-source 552 00:30:46,899 --> 00:30:50,139 and I know that with 3d printing one the 553 00:30:50,139 --> 00:30:53,399 things with it just happened to sort of gloom has 554 00:30:53,399 --> 00:30:58,179 hands were falling out of there time-line do you know if the current 555 00:30:58,179 --> 00:30:58,620 round 556 00:30:58,620 --> 00:31:02,570 I'll tools are actually coming out of hand right now or is this going to be an 557 00:31:02,570 --> 00:31:03,340 issue where 558 00:31:03,340 --> 00:31:07,240 the patent like anything there's there's always issues with IP 559 00:31:07,240 --> 00:31:11,429 one of the reasons why we chose to a target feel a sin for our 560 00:31:11,429 --> 00:31:15,529 are wet where kit was it deletion is a naturally-occurring compound 561 00:31:15,529 --> 00:31:18,620 it's not something that was invented by somebody that would be under 562 00:31:18,620 --> 00:31:22,260 IP constraints om that yet that is a problem 563 00:31:22,260 --> 00:31:26,370 and really no one knows what to do there's a couple 564 00:31:26,370 --> 00:31:30,419 there's a issue love a tool called genome compiler which is a 565 00:31:30,419 --> 00:31:35,240 DNA design tool calm and the had gooey the allergy to 566 00:31:35,240 --> 00:31:38,960 organize your your genetic code om and they actually 567 00:31:38,960 --> 00:31:43,600 were hit with a patent infringement case from teeny 2.0 saying that we own 568 00:31:43,600 --> 00:31:46,940 the the patent on using a gooey 569 00:31:46,940 --> 00:31:52,149 to manipulate a text file and the I mean somebody gave a net Patton and the 570 00:31:52,149 --> 00:31:55,740 had to come to an agreement and that's unfortunate 571 00:31:55,740 --> 00:31:58,539 but the good news times don't last forever and there's there's think 572 00:31:58,539 --> 00:31:59,919 there's a there's a keypad 573 00:31:59,919 --> 00:32:03,159 is a keypad for DNA synthesis I believe that 574 00:32:03,159 --> 00:32:06,299 as a DNA synthesis is a patented process right now 575 00:32:06,299 --> 00:32:11,230 but wanted to keep at Cinespace is set to expire in 2014 or 2015 576 00:32:11,230 --> 00:32:15,510 I'm so it's going to be exciting to see some of the smaller groups that maybe 577 00:32:15,510 --> 00:32:17,649 had the technology available but 578 00:32:17,649 --> 00:32:20,700 I you can't bring it out for 579 00:32:20,700 --> 00:32:23,760 for IP fears Tim soon will be able to do so 580 00:32:23,760 --> 00:32:28,090 keep keep your eye on a would be too careful there about the possibility of 581 00:32:28,090 --> 00:32:29,350 miss you so mean 582 00:32:29,350 --> 00:32:33,610 a bad guy to kidnap you June take you to an evil lab somewhere but 583 00:32:33,610 --> 00:32:37,779 when everyone asked about his the who's a piece on as it happens go camera that 584 00:32:37,779 --> 00:32:38,470 the 585 00:32:38,470 --> 00:32:41,770 are the new new genetic alphabet letters with 586 00:32:41,770 --> 00:32:45,850 know the promise ring see ATG home porn do you think that action why in a 587 00:32:45,850 --> 00:32:47,190 concept is going to be 588 00:32:47,190 --> 00:32:51,029 well that really change things I think you will I think it's in this an example 589 00:32:51,029 --> 00:32:54,500 love how malleable life is and how malleable these 590 00:32:54,500 --> 00:32:58,289 self-replicating by owning a robots are if we can 591 00:32:58,289 --> 00:33:01,450 add extra pieces to it have them still work maybe does it make 592 00:33:01,450 --> 00:33:04,700 the codes more expressive to have two extra characters I don't know 593 00:33:04,700 --> 00:33:08,260 are you coming out right here are you going on a rule sarisi are you going to 594 00:33:08,260 --> 00:33:10,200 go down that road alone try to 595 00:33:10,200 --> 00:33:14,110 child-care yeah I mean party like synthetic biology assaults to you 596 00:33:14,110 --> 00:33:17,820 I learn how these fundamental organisms work by rebuilding them from scratch 597 00:33:17,820 --> 00:33:18,730 kinda thing 598 00:33:18,730 --> 00:33:22,119 so you know that's that's a very good example you know maybe 599 00:33:22,119 --> 00:33:25,919 eons ago in the first organisms they had like a dozen different kinds bases and 600 00:33:25,919 --> 00:33:29,460 this year too that were there and now they're not there's something back 601 00:33:29,460 --> 00:33:32,740 its its sole really really cool and I'm 602 00:33:32,740 --> 00:33:35,020 yet you know he can think at times according up visions whenever is the 603 00:33:35,020 --> 00:33:36,980 same as 604 00:33:36,980 --> 00:33:43,279 paint on the team i kidnap you okay 605 00:33:43,279 --> 00:33:48,190 I I'm I was wondering how difficult do you think it would be too 606 00:33:48,190 --> 00:33:51,429 so to create I think arbitrary pathway 607 00:33:51,429 --> 00:33:55,139 so like an arbitrary metabolic pathways are discovering one in nature 608 00:33:55,139 --> 00:33:59,260 you find some product some protein product that you wanna synthesize 609 00:33:59,260 --> 00:34:03,830 and you just sorta get the sequence from that mean you inject the sequence 610 00:34:03,830 --> 00:34:07,760 in one of these sorta tabletop systems like is that as a possible 611 00:34:07,760 --> 00:34:11,889 yep exactly pretty much need yeah exactly describe to you find sequences 612 00:34:11,889 --> 00:34:12,800 and then you can 613 00:34:12,800 --> 00:34:16,530 on the specimen they could be you know the just for us a person's was just low 614 00:34:16,530 --> 00:34:19,810 or they could be enzymes that's you know converts I am 615 00:34:19,810 --> 00:34:24,190 sugar into some my way see sir I'm that's what I was a kid is 616 00:34:24,190 --> 00:34:27,589 that so I i guess i reset the wrong way I mean what are the whether restrictions 617 00:34:27,589 --> 00:34:28,069 on my 618 00:34:28,069 --> 00:34:31,290 arbitrary pathways that for example there's some bacteria that 619 00:34:31,290 --> 00:34:35,250 that do electron transport right they just process electron so 620 00:34:35,250 --> 00:34:39,659 yeah presumably like you could use that make us our class a computer right by 621 00:34:39,659 --> 00:34:43,919 growing bacteria netting pathways to them 622 00:34:43,919 --> 00:34:49,720 bad works that way rates Utah commitment to giving the up-front transport 623 00:34:49,720 --> 00:34:54,129 Jasola creating an alternate chain so that you can actually create logic gates 624 00:34:54,129 --> 00:34:55,240 with an 625 00:34:55,240 --> 00:34:58,420 bring cultures bacteria yeah exactly at what 626 00:34:58,420 --> 00:35:01,950 it's totally that's that's a good thats synthetic biology is 627 00:35:01,950 --> 00:35:05,700 creating is sort of systems that and to do these kinds of things 628 00:35:05,700 --> 00:35:09,650 and as far as and yeah I mustered I'm 629 00:35:09,650 --> 00:35:13,170 maybe it didn't quite get a question cell but Dr freeing our pitchers this is 630 00:35:13,170 --> 00:35:13,910 like that is 631 00:35:13,910 --> 00:35:18,369 see a you can definitely do that maybe eight you know where things go in the 632 00:35:18,369 --> 00:35:19,300 cell and 633 00:35:19,300 --> 00:35:22,319 they have their own system setup and so ideally when you're trying to freeze a 634 00:35:22,319 --> 00:35:25,300 drug you on tap into this is an important resource from it 635 00:35:25,300 --> 00:35:28,520 and Tino even on that level then you can manipulate 636 00:35:28,520 --> 00:35:33,940 its to enhance isn't I wasn't in the direction of your drug pathway basically 637 00:35:33,940 --> 00:35:38,820 thank you 638 00:35:38,820 --> 00:35:41,830 are I'm so I was a big hug with the 639 00:35:41,830 --> 00:35:46,300 current state of the art is a for flexibility in creating weird switzer 640 00:35:46,300 --> 00:35:49,810 Park organisms and jeans like do you need 641 00:35:49,810 --> 00:35:53,920 the gene to be in somebody's catalog user through the arbitrary sequences up 642 00:35:53,920 --> 00:35:54,910 between you know what 643 00:35:54,910 --> 00:35:59,690 either or I mean you can right now he just like very much letters like that 644 00:35:59,690 --> 00:36:00,960 like water on Lake I 645 00:36:00,960 --> 00:36:04,410 you know ittt diskettes incise I'm you may not do anything 646 00:36:04,410 --> 00:36:07,470 bites on you know there's a chance it would do anything 647 00:36:07,470 --> 00:36:11,150 I'm will ensure that but see that can't you get 648 00:36:11,150 --> 00:36:14,830 the arbitrary surfaces supported a delight for yeah I mean you can look up 649 00:36:14,830 --> 00:36:16,660 online to find the guy with the sequence for 650 00:36:16,660 --> 00:36:21,270 party even say like order and deal just sent you an email you put any bacterial 651 00:36:21,270 --> 00:36:21,650 glow 652 00:36:21,650 --> 00:36:24,950 and time there's one thing that we didn't discuss it so we've been talking 653 00:36:24,950 --> 00:36:25,320 about 654 00:36:25,320 --> 00:36:28,400 in a purely by like coding for purely biological functions 655 00:36:28,400 --> 00:36:32,140 you can code for logical functions as well because if deion DNA is a 656 00:36:32,140 --> 00:36:36,369 information storage medium you could translate a poem 657 00:36:36,369 --> 00:36:41,310 into DNA code representation and put that DNA code into an organism 658 00:36:41,310 --> 00:36:45,470 and then have that organism live immune if you put in the right place so doesn't 659 00:36:45,470 --> 00:36:46,240 kill it 660 00:36:46,240 --> 00:36:50,609 a bit with live in replicating grow NBC what you've got is a machine 661 00:36:50,609 --> 00:36:54,839 that makes copies of that poem and it exponentially increases the number of 662 00:36:54,839 --> 00:36:55,250 poems 663 00:36:55,250 --> 00:36:58,290 this has been something that sorry done by Keane poet I 664 00:36:58,290 --> 00:37:02,160 a number of years ago I'm but that that some again so we can touch on that there 665 00:37:02,160 --> 00:37:02,599 are 666 00:37:02,599 --> 00:37:05,599 are logical uses 14 is a 667 00:37:05,599 --> 00:37:08,730 information storage medium that has nothing to do with life yet 668 00:37:08,730 --> 00:37:12,099 DNA is like really really stable if set room temperatures that people are 669 00:37:12,099 --> 00:37:13,170 bringing stuff like 670 00:37:13,170 --> 00:37:16,540 shakespearian Mozart and according to DNA just for long-term storage 671 00:37:16,540 --> 00:37:20,010 anything and that's that's that's typical that that should write that 672 00:37:20,010 --> 00:37:20,440 extra 673 00:37:20,440 --> 00:37:27,089 such as crazy thank you so 674 00:37:27,089 --> 00:37:31,240 your made the arm pretty common analogy that both the genomes and computer 675 00:37:31,240 --> 00:37:32,160 programs are code 676 00:37:32,160 --> 00:37:35,960 number computer programs are usually written in a high-level language that's 677 00:37:35,960 --> 00:37:38,270 readable by humans with a well-defined semantics 678 00:37:38,270 --> 00:37:41,710 so is there anything like that 679 00:37:41,710 --> 00:37:45,770 forward the the code for genome or is that a misleading analogy there 680 00:37:45,770 --> 00:37:49,109 or could it be possible when you begin to develop something like that 681 00:37:49,109 --> 00:37:53,839 I think it's definitely possible com a lot of work needs to be done so 682 00:37:53,839 --> 00:37:57,089 again in biology we're not working for first from first principles 683 00:37:57,089 --> 00:38:02,119 in computing we r we made computers we know exactly with the microcode is every 684 00:38:02,119 --> 00:38:06,140 we build up whereas work predicting down with bio and so we're not at the point 685 00:38:06,140 --> 00:38:06,839 where we 686 00:38:06,839 --> 00:38:10,160 really know exactly that the first principles and 687 00:38:10,160 --> 00:38:13,630 and I don't know if we ever will on but that you know to be 688 00:38:13,630 --> 00:38:18,260 actual bioengineering we're going to have to get down to that level and a 689 00:38:18,260 --> 00:38:22,599 we're not there yet yet so I think maybe you're talking about the annotated 690 00:38:22,599 --> 00:38:23,980 genomes and 691 00:38:23,980 --> 00:38:27,630 you need to see what price do what anything in that that exists I'm 692 00:38:27,630 --> 00:38:30,670 it's not a very you know user 693 00:38:30,670 --> 00:38:33,930 friendly people readable kinda thing letting go to see the icing on the cake 694 00:38:33,930 --> 00:38:34,599 this genome 695 00:38:34,599 --> 00:38:37,890 and initially on sequences I'm butts 696 00:38:37,890 --> 00:38:40,980 you know it needs to be a better interface hands 697 00:38:40,980 --> 00:38:44,970 know hopefully somebody's gonna do that in hit and also when 698 00:38:44,970 --> 00:38:48,250 you know when you're writing suffering a computer and you wanna test your 699 00:38:48,250 --> 00:38:52,040 your code you run it you execute rate Nico where's 700 00:38:52,040 --> 00:38:55,670 if you're testing your biological code you have to get the DNA synthesized 701 00:38:55,670 --> 00:38:56,140 Scott 702 00:38:56,140 --> 00:38:59,510 which cost money you have to transfixed the organism which takes time 703 00:38:59,510 --> 00:39:02,890 if the girl the organism which takes time then you have to be able to look 704 00:39:02,890 --> 00:39:06,839 and characterize to see what's actually happening so that that the 705 00:39:06,839 --> 00:39:10,290 that iteration process in bio coding 706 00:39:10,290 --> 00:39:13,480 is right now a lot longer then software 707 00:39:13,480 --> 00:39:19,450 yes it's a taxiway formal methods is one of the oldest outfield the computer 708 00:39:19,450 --> 00:39:21,310 science that what you just described so 709 00:39:21,310 --> 00:39:24,359 awesome are great and if the guy 710 00:39:24,359 --> 00:39:27,970 right I'm still love will head to the lobby or something that'll 711 00:39:27,970 --> 00:39:34,970 was not marked thank you 712 00:40:21,190 --> 00:40:24,700 check check check check 713 00:40:24,700 --> 00:40:28,070 a good evening ladies and gentlemen these social engineering panel they are 714 00:40:28,070 --> 00:40:29,650 all waiting for will be happening 715 00:40:29,650 --> 00:40:32,910 in just a moment up I want to let you know that their is 716 00:40:32,910 --> 00:40:37,050 a chip tens concert happening right now at ten o'clock are probably going on at 717 00:40:37,050 --> 00:40:38,440 eleven o'clock urbanites 718 00:40:38,440 --> 00:40:43,440 I am so if you're leaving please go check that out its gonna be a lot of fun 719 00:40:43,440 --> 00:40:47,580 are then also if you are over here if you're in one of the Isles 720 00:40:47,580 --> 00:40:52,070 in your camping out the security crew is clearing that out right now you're 721 00:40:52,070 --> 00:40:55,030 years I think you can be all the way up against the wall are right along the 722 00:40:55,030 --> 00:40:57,280 edge right there but you can be blocking I always 723 00:40:57,280 --> 00:41:01,190 so please I hope the security crew can can you get a huge round of applause and 724 00:41:01,190 --> 00:41:03,380 security guys have a really rough job 725 00:41:03,380 --> 00:41:10,380 thank you so much for that so you don't forget the chiptunes concert tonight 726 00:41:13,120 --> 00:41:15,910 happening right now until probably around 11 midnight's 727 00:41:15,910 --> 00:41:19,620 and the social engineering panel will be up in just a few moments 728 00:41:19,620 --> 00:41:26,620 thank you 729 00:41:59,830 --> 00:42:00,120 host